產(chǎn)品編號 | bs-14596R-BF594 |
英文名稱 | Rabbit Anti-ENO4/BF594 Conjugated antibody |
中文名稱 | BF594標記的ENO4蛋白抗體 |
別 名 | C10orf134; AC023283.3; C10orf134; eno4; ENO4_HUMAN; Enolase 4; Enolase family member 4; Enolase-like protein ENO4. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 信號轉導 新陳代謝 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat, (predicted: Human, Cow, Rabbit, Sheep, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 69kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ENO4 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, alpha enolase, beta enolase and gamma enolase. alpha enolase expression has been detected on most tissues, whereas betaenolase is expressed predominantly in muscle tissue and gamma enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. ENO4 (Enolase-like protein ENO4), also known as C10orf134, is a 628 amino acid protein that belongs to the Enolase family. ENO4 is expressed as two isoforms produced by alternative splicing and is encoded by a gene mapping to human chromosome 10q25.3 and murine chromosome 19 D3. Similarity: Belongs to the enolase family. Database links: Entrez Gene: 387712 Human Entrez Gene: 226265 Mouse Omim: 131375 Human SwissProt: A6NNW6 Human SwissProt: Q8C042 Mouse Unigene: 696001 Human Unigene: 103154 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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