產(chǎn)品編號 | bs-4265R-RBITC |
英文名稱 | Rabbit Anti-E2F8/RBITC Conjugated antibody |
中文名稱 | 羅丹明(RBITC)標記的轉錄因子E2F8抗體 |
別 名 | E2F 8; E2F family member 82; E2F transcription factor 8; E2F-8; E2f8; E2F8_HUMAN; FLJ23311; Transcription factor E2F8. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 免疫學 染色質和核信號 信號轉導 轉錄調節(jié)因子 新陳代謝 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse, Rat, (predicted: Human, Chicken, Pig, Horse, Rabbit, ) |
產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 94kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human E2F8 (285-326aa) |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]. Function: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Subunit: Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is important for DNA binding. Subcellular Location: Nucleus. Similarity: Belongs to the E2F/DP family Database links: Entrez Gene: 79733 Human Entrez Gene: 108961 Mouse Omim: 612047 Human SwissProt: A0AVK6 Human SwissProt: Q58FA4 Mouse Unigene: 523526 Human Unigene: 240566 Mouse Unigene: 395068 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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