產(chǎn)品編號 | bs-7770R-HRP |
英文名稱 | Rabbit Anti-SEPT10/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的細胞分化蛋白SEPT10抗體 |
別 名 | SEPT 10; SEPT-10; Septin 10; Septin-10; Septin 10; SEP10_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 細胞周期蛋白 細胞分化 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse, (predicted: Human, Rat, Chicken, Pig, Horse, Rabbit, ) |
產(chǎn)品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 53kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SEPT10 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The SEPT10 gene encodes a member of the septin family of cytoskeletal proteins which have GTPase activity. This protein localizes to the cytoplasm and nucleus and displays GTP-binding and GTPase activity. Alternate splicing results in two transcript variants encoding different isoforms. Function: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). Subunit: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton (By similarity). Note=Using a GFP-fusion protein, detected in the nucleus (PubMed:12711328). Tissue Specificity: Widely expressed. Abundantly expressed in heart and kidney, placenta, skeletal muscles, liver and lung, as well as various tumor cell lines. Similarity: Belongs to the septin family. Database links: Entrez Gene: 151011 Human Entrez Gene: 103080 Mouse Omim: 611737 Human SwissProt: Q9P0V9 Human SwissProt: Q8C650 Mouse Unigene: 469615 Human Unigene: 346842 Mouse Unigene: 397041 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. SEPTIN(SEPT)家族是細胞骨架蛋白的一種,具有GTPase的活性。經(jīng)研究發(fā)現(xiàn),SEPT參與細胞核分裂、細胞膜的運輸以及構成細胞骨架。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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