產(chǎn)品編號 | bs-0062R-BF350 |
英文名稱 | Rabbit Anti-Calretinin/BF350 Conjugated antibody |
中文名稱 | BF350標記的鈣結(jié)合蛋白抗體 |
別 名 | 29 kDa calbindin; CAB 29; CAB29; CAL 2; CAL2; CALB 2; CALB2; Calbindin 2 29kDa; Calbindin 2; Calbindin D29K; Calbindin2; CR; CALB2_HUMAN; Calretinin. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 神經(jīng)生物學 結(jié)合蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) |
產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 29kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Calretinin |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Calretinin is a calcium-binding protein which is abundant in auditory neurons. It belongs to the calbindin family. Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin1. Function: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Tissue Specificity: Brain. Similarity: Belongs to the calbindin family. Contains 6 EF-hand domains. Database links: Entrez Gene: 396255 Chicken Entrez Gene: 794 Human Entrez Gene: 12308 Mouse Entrez Gene: 393684 Zebrafish Omim: 114051 Human SwissProt: P07090 Chicken SwissProt: P22676 Human SwissProt: Q08331 Mouse Unigene: 106857 Human Unigene: 2755 Mouse Unigene: 10321 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 鈣結(jié)合蛋白(Calretinin,CR)主要存在于神經(jīng)組織中,近10%的腺癌可以表達此蛋白的局部染色陽性,而絕大多數(shù)的間皮瘤為陽性表達,因此它是診斷間皮瘤的一個有用的標記物 。 鈣結(jié)合蛋白是平滑肌和非肌性調(diào)控蛋白。它可以與肌動蛋白、肌球蛋白、原肌球蛋白和鈣調(diào)素相互作用。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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