產(chǎn)品編號 | bs-1557G-BF750 |
英文名稱 | HBsAg, BF750 conjugated |
中文名稱 | BF750標記的山羊抗人乙肝表面抗原抗體 |
別 名 | L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; S; HBSAG_HBVC5. |
研究領(lǐng)域 | 微生物學 細菌及病毒 |
抗體來源 | Goat |
克隆類型 | Polyclonal |
交叉反應 | |
產(chǎn)品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 44kDa |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant HBsAg protein |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. SWISS: P03140 Gene ID: 944569 Database links: Entrez Gene: 2828293 Hepatitis B virus Entrez Gene: 944569 Hepatitis B virus SwissProt: P03138 Hepatitis B virus SwissProt: P03140 Hepatitis B virus SwissProt: P03141 Hepatitis B virus SwissProt: Q81157 Hepatitis B virus SwissProt: Q81158 Hepatitis B virus SwissProt: Q81159 Hepatitis B virus SwissProt: Q81160 Hepatitis B virus |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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