| 產(chǎn)品編號 | bsm-43099M |
| 英文名稱 | PRL Mouse mAb |
| 中文名稱 | 泌乳素單克隆抗體 |
| 別 名 | Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 生長因子和激素 激酶和磷酸酶 內(nèi)分泌病 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1H7 |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PRL protein: 29-227/227 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. SWISS: P01236 Gene ID: 5617 Database links: Entrez Gene: 5617 Human Entrez Gene: 19109 Mouse Omim: 176760 Human SwissProt: P01236 Human SwissProt: P06879 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Pituitary tissue lysates
Primary: Anti-PRL (bsm-43099M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa
2688號盒-20230329-LXH_25.9x-2-B.jpg)
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL) Monoclonal Antibody, Unconjugated (bsm-43099M) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
2674號盒-20230316-WDD_40.0x-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL ) Monoclonal Antibody, Unconjugated (bsm-43099M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
