| 產(chǎn)品編號 | bs-22199R |
| 英文名稱 | MyoD1/Myf3 Rabbit pAb |
| 中文名稱 | 肌原調(diào)節(jié)蛋白抗體 |
| 別 名 | Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3. |
| 研究領域 | 免疫學 發(fā)育生物學 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MyoD1/Myf3 : 31-130/318 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] Function: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus; which is required for proteasomal degradation Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P15172 Gene ID: 4654 Database links: Entrez Gene: 374048 Chicken Entrez Gene: 4654 Human Entrez Gene: 17927 Mouse Entrez Gene: 443405 Sheep Omim: 159970 Human SwissProt: P16075 Chicken SwissProt: P15172 Human SwissProt: P10085 Mouse SwissProt: P29331 Sheep Unigene: 181768 Human Unigene: 1526 Mouse Unigene: 9493 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Skeletal muscle Lysates
Lane 2: Mouse Tongue Lysates
Lane 3: Mouse NIH/3T3 cell Lysates
Lane 4: Mouse Raw264.7 cell Lysates
Lane 5: Rat Skeletal muscle Lysates
Lane 6: Human HL-60 cell Lysates
Lane 7: Human K562 cell Lysates
Primary: Anti-MyoD1Myf3 (bs-22199R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35kDa
Observed band size: 50kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
