| 產(chǎn)品編號 | bs-23062R |
| 英文名稱 | Involucrin Rabbit pAb |
| 中文名稱 | 囊包蛋白/內(nèi)披蛋白抗體 |
| 別 名 | INVO_MOUSE; involucrin; ivl. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞周期蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Involucrin: 21-120/467 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]. Function: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Subunit: Directly or indirectly cross-linked to cornifelin (CNFN). Subcellular Location: Cytoplasm. Tissue Specificity: Keratinocytes of epidermis and other stratified squamous epithelia. Post-translational modifications: Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. Similarity: Belongs to the involucrin family. SWISS: P48997 Gene ID: 16447 Database links: Entrez Gene: 3713 Human Entrez Gene: 16447 Mouse Omim: 147360 Human SwissProt: P07476 Human SwissProt: P48997 Mouse Unigene: 516439 Human Unigene: 207365 Mouse Unigene: 142591 Rat 套膜蛋白又稱內(nèi)披蛋白或囊包蛋白，與毛透明蛋白共同構(gòu)成角質(zhì)細胞包膜支架的蛋白質(zhì)。在角質(zhì)細胞分化時，首先出現(xiàn)在表皮棘層的上層細胞中的標志性蛋白質(zhì)，該蛋白在角質(zhì)細胞和其他復(fù)層扁平上皮合成的蛋白。最初存在于細胞質(zhì)中，然后通過轉(zhuǎn)谷氨酰胺酶的作用，轉(zhuǎn)變?yōu)榻宦?lián)的膜蛋白。 |
| 產(chǎn)品圖片 |
Sample:
MCM-7(Human) Cell Lysate at 30 ug
Primary: Anti-Involucrin (bs-23062R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
