| 產(chǎn)品編號 | bs-8635R |
| 英文名稱 | Phostensin Rabbit pAb |
| 中文名稱 | 蛋白磷酸酶1調(diào)節(jié)亞單位18抗體 |
| 別 名 | HKMT1098; KIAA1949; PPP1R18; Protein phosphatase 1 F actin cytoskeleton targeting subunit; Protein phosphatase 1 regulatory subunit 18. |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 結合蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Phostensin: 101-200/613 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Protein phosphatase-1 (PP1; see MIM 176875) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin (see MIM 102610) cytoskeleton (Kao et al., 2007 [PubMed 17374523]).[supplied by OMIM, Mar 2008] Subunit: Interacts with Protein phosphatase 1 (PP1). Subcellular Location: Cytoplasm; cytoskeleton. SWISS: Q6NYC8 Gene ID: 170954 Database links: Entrez Gene: 100051083 Horse Entrez Gene: 170954 Human Omim: 610990 Human SwissProt: Q6NYC8 Human Unigene: 101150 Human |
| 產(chǎn)品圖片 |
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti- Phostensin (bs-8635R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 63 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
