| 產(chǎn)品編號 | bs-19275R |
| 英文名稱 | Nkx2.6 Rabbit pAb |
| 中文名稱 | 轉(zhuǎn)錄因子同源蛋白Nkx2.6抗體 |
| 別 名 | CSX2; Homeobox protein NK-2 homolog F; homeobox protein NK2 homolog F; Homeobox protein Nkx-2.6; Homeobox protein Nkx2.6; NK2 homeobox 6; NK2 transcription factor related, locus 6(Drosophila); NKX2-6; NKX26_HUMAN; NKX2F; NKX4 2; Tinman paralog. |
| 研究領(lǐng)域 | 心血管 細胞生物 發(fā)育生物學 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nkx2.6: 1-100/301 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a homeobox-containing protein that belongs to the NK-2 homeobox family. This protein is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are associated with persistent truncus arteriosus.[provided by RefSeq, Aug 2011] Function: In conjunction with NKX2-5, may play a role in both pharyngeal and cardiac embryonic development. Subcellular Location: Nucleus DISEASE: [MIM:217095]: A group of congenital heart defects involving the outflow tracts. Examples include truncus arteriosus communis, double-outlet right ventricle and transposition of great arteries. Truncus arteriosus communis is characterized by a single outflow tract instead of a separate aorta and pulmonary artery. In transposition of the great arteries, the aorta arises from the right ventricle and the pulmonary artery from the left ventricle. In double outlet of the right ventricle, both the pulmonary artery and aorta arise from the right ventricle. Similarity: Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: A6NCS4 Gene ID: 137814 Database links:
Entrez Gene: 137814 Human
Entrez Gene: 18092 Mouse
Omim: 611770 Human
SwissProt: A6NCS4 Human
SwissProt: P43688 Mouse
Unigene: 532654 Human
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| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nkx2.6) Polyclonal Antibody, Unconjugated (bs-19275R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
