| 產(chǎn)品編號 | bs-17649R |
| 英文名稱 | SPTBN3 Rabbit pAb |
| 中文名稱 | 血影蛋白β3/spectrin β IV抗體 |
| 別 名 | Spectrin beta chain, brain 3; beta-IV spectrin; brain 3; KIAA1642; non-erythroid beta chain 3; QV; Spectrin; Spectrin beta chain; spectrin, beta, non-erythrocytic 4; spectrin, non-erythroid beta chain 3; SPNB4; SPTBN3; SPTBN4; SPTN4_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 289 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPTBN3: 2401-2500/2564 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous Alpha and beta chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta IV is a non-erythrocytic member of the beta-spectrin family. It is expressed in brain and pancreatic islets and localizes to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Spectrin beta IV is a 2,564 amino acid protein with four isoforms due to alternative splicing events. Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Tissue Specificity: Abundantly expressed in brain and pancreatic islets. Similarity: Belongs to the spectrin family. Contains 2 CH (calponin-homology) domains. Contains 1 PH domain. Contains 18 spectrin repeats. SWISS: Q9H254 Gene ID: 57731 Database links: Entrez Gene: 57731 Human Omim: 606214 Human SwissProt: Q9H254 Human Unigene: 32706 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPTBN3) Polyclonal Antibody, Unconjugated (bs-17649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
