| 產(chǎn)品編號 | bs-17284R |
| 英文名稱 | SCGF Rabbit pAb |
| 中文名稱 | 干細胞生長因子SCGF抗體 |
| 別 名 | SCGF beta; C type lectin domain family 11 member A; C type lectin superfamily member 3; C-type lectin domain family 11 member A; C-type lectin superfamily member 3; CLC11_HUMAN; CLEC11A; CLECSF3; LSLCL; Lymphocyte secreted C type lectin; Lymphocyte secreted C-type lectin; lymphocyte secreted long form of C type lectin; p47; Stem cell growth factor; stem cell growth factor lymphocyte secreted C type lectin. |
| 研究領域 | 細胞生物 干細胞 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SCGF: 101-200/323 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SCGF is a cytokine for primitive hematopoietic progenitor cells and exhibits species-specific activity. It is predominantly expressed in proliferating chondrocytes, bone marrow cells, the periosteum and the perichondrium of skeletal tissues. SCGF contains an N-terminal Arg-Gly-Asp triplet (RGD triplet), leucine zipper domains, a C-terminal C-type lectin domain and numerous potential glycosylation sites. It has burst-promoting activity and granulocyte/macrophage colony-promoting activity in vitro. SCGF is directly correlated to granulocyte recovery following stem cell transplantation and thus can be an indicator of hematopoietic recovery following stem cell transplantation. Function: Stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. Acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. Suppresses SCF-stimulated erythrocyte proliferation. Subcellular Location: Cytoplasm. Secreted. Tissue Specificity: Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver. Post-translational modifications: O-glycosylated. Probably sulfated on the O-glycans. Similarity: Contains 1 C-type lectin domain. SWISS: Q9Y240 Gene ID: 6320 Database links: Entrez Gene: 6320 Human Omim: 604713 Human SwissProt: Q9Y240 Human Unigene: 512680 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCGF) Polyclonal Antibody, Unconjugated (bs-17284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
