| 產(chǎn)品編號 | bs-10656R |
| 英文名稱 | PI3 Kinase p110 delta Rabbit pAb |
| 中文名稱 | 磷脂酰肌醇激酶催化亞單位D抗體 |
| 別 名 | p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform; phosphoinositide 3 |
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Specific References (2) | bs-10656R has been referenced in 2 publications.
[IF=6.656] Fangbo Zhang. et al. Huashibaidu Formula Attenuates Sepsis-Induced Acute Lung Injury via Suppressing Cytokine Storm: Implications for Treatment of COVID-19. PHYTOMEDICINE. 2022 Nov;:154549 WB ; Mouse.
[IF=5.561] Minrui Wu. et al. The Effect and Mechanism of Corilagin from Euryale Ferox Salisb Shell on LPS-Induced Inflammation in Raw264.7 Cells. FOODS. 2023 Jan;12(5):979 WB ; Mouse.
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| 研究領域 | 腫瘤 免疫學 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 115 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PI3 Kinase p110 delta: 51-150/1044 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |
Sample: Thymus (Mouse) Lysate at 40 ug
Primary: Anti-PI3 Kinase p110 delta (bs-10656R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Sample:
Lane 1: Human HT-1080 cell lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-PI3 Kinase p110 delta (bs-10656R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 120 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated(bs-10656R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
