產品編號 | bs-14058R |
英文名稱 | CRISP1 Rabbit pAb |
中文名稱 | 富含半胱氨酸分泌蛋白1抗體 |
別 名 | Acidic epididymal glycoprotein homolog; Acidic epididymal glycoprotein like 1; AEG like protein; AEG related protein; AEG-like protein; AEGL1; ARP; CRIS1_HUMAN; CRISP-1; CRISP1; Cysteine rich secretory protein 1; Cysteine rich secretory protein 1 delta; Cysteine-rich secretory protein 1; HSCRISP1D; HSCRISP1G; HUMARP; Protein DE like. |
研究領域 | 細胞生物 發(fā)育生物學 信號轉導 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 26 kDa |
檢測分子量 | |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CRISP1: 21-120/249 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head, where it plays a role in sperm-egg fusion. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] Function: May have a role in sperm-egg fusion and maturation. Subcellular Location: Note=Located in the lumen and epithelium of distal ductus efferentes and epididymal ducts, and on the postacrosomal region of the sperm head. Tissue Specificity: Caput, corpus, and cauda regions of the epididymis, the ductus deferens, sperm and seminal plasma. Similarity: Belongs to the CRISP family. Contains 1 SCP domain. Contains 1 ShKT domain. SWISS: P54107 Gene ID: 167 Database links: Entrez Gene: 167 Human Omim: 601193 Human SwissProt: P54107 Human Unigene: 109620 Human |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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