| 產(chǎn)品編號 | bs-13239R |
| 英文名稱 | FZD3 Rabbit pAb |
| 中文名稱 | 卷曲蛋白3抗體 |
| 別 名 | Frizzled 3; Frizzled 3 seven transmembrane spanning receptor; Frizzled family receptor 3; Frizzled homolog 3(Drosophila); Frizzled homolog 3; Frizzled-3; Frizzled3; Fz 3; Fz-3; Fz3; FZD 3; Fzd3; FZD3_HUMAN; hFz 3; hFz3. |
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Specific References (1) | bs-13239R has been referenced in 1 publications.
[IF=2.64] Su, Lin, et al. "Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease." Experimental and Molecular Pathology(2016). IHC-P ; Human.
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| 研究領域 | 細胞生物 信號轉導 干細胞 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FZD3 protein: 23-205/666 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010] Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Subunit: Interacts with VANGL2. Subcellular Location: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. Post-translational modifications: Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. Similarity: Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain. SWISS: Q9NPG1 Gene ID: 7976 Database links: Entrez Gene: 7976 Human Entrez Gene: 14365 Mouse Entrez Gene: 100339544 Rabbit Entrez Gene: 395572 Chicken Entrez Gene: 100054732 Horse Omim: 606143 Human SwissProt: Q9NPG1 Human SwissProt: Q61086 Mouse Unigene: 40735 Human Unigene: 214687 Mouse Unigene: 404299 Mouse Unigene: 178695 Rat |
| 產(chǎn)品圖片 |
Protein: pancreas(mouse) lysates at 30ug;
Primary: Anti-FZD3 (bs-13239R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size : 74kD
Observed band size : 74kD
Protein: brain(Rat) lysates at 30ug;
Primary: Anti-FZD3 (bs-13239R) at 1:300;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 74kD
Observed band size : 74kD, 25kD
Sample:
Lane 1: Human SW480 cell Lysates
Lane 2: Human U-2 OS cell Lysates
Lane 3: Human A431 cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Primary: Anti-FZD3 (bs-13239R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74kDa
Observed band size: 70kDa
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
