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PSMA4 Rabbit pAb (bs-12260R)  
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產(chǎn)品編號(hào) bs-12260R
英文名稱 PSMA4 Rabbit pAb
中文名稱 蛋白酶體PSMα4/20S Proteasome α4抗體
別    名 HC9; HsT17706; Macropain subunit C9; MGC111191; MGC12467; MGC24813; Multicatalytic endopeptidase complex subunit C9; Proteasome(prosome macropain) subunit alpha type 4; Proteasome alpha 4 subunit; Proteasome component C9; Proteasome subunit alpha type 4; Proteasome subunit alpha type-4; Proteasome subunit HC9; Proteasome subunit L; PSA4_HUMAN; PSC9; PSMA 4; psmA4.  
研究領(lǐng)域 細(xì)胞生物  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig,Horse,Monkey)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human PSMA4: 161-261/261 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The proteasome represents a large protein complex that exists inside all eukaryotes and archaea, and in some bacteria. The main function of proteasomes is to degrade unnecessary or damaged proteins by proteolysis. The most common form of the proteasome, known as the 26S Proteasome, contains one 20S Proteasome core particle structure and two 19S regulatory caps. The 20S Proteasome core is hollow and forms an enclosed cavity, where proteins are degraded, as well as openings at the two ends to allow the target protein to enter. The 20S Proteasome core particle contains many subunits, depending on the organism. All of the subunits fall into one of two types: alpha subunits, which are structural, serve as docking domains for the regulatory particles and exterior gates blocking unregulated access to the interior cavity; or beta subunits, which are predominantly catalytic. The outer two rings in the proteasome consist of seven ?subunits each, and the inner two rings each consist of seven beta subunits.

Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Belongs to the peptidase T1A family.

SWISS:
P25789

Gene ID:
5685

Database links:

Entrez Gene: 5685 Human

Entrez Gene: 26441 Mouse

Entrez Gene: 29671 Rat

Omim: 176846 Human

SwissProt: P25789 Human

SwissProt: Q9R1P0 Mouse

SwissProt: P21670 Rat

Unigene: 251531 Human

Unigene: 30270 Mouse

Unigene: 440582 Mouse

Unigene: 11076 Rat



產(chǎn)品圖片
Sample: kidney (mouse) Lysate at 40 ug Primary: Anti- PSMA4(bs-12260R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PSMA4 Polyclonal Antibody, Unconjugated(bs-12260R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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