產(chǎn)品編號(hào) | bs-7112R |
英文名稱 | Rabbit Anti-EDG1 antibody |
中文名稱 | 內(nèi)皮細(xì)胞分化鞘脂G蛋白偶聯(lián)受體1抗體 |
別 名 | CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; G protein coupled sphingolipid receptor; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; S1PR1_HUMAN; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1. |
![]() |
Specific References (3) | bs-7112R has been referenced in 3 publications.
[IF=6.117] Arianna Mazzoli. et al. SKELETAL MUSCLE INSULIN RESISTANCE AND ADIPOSE TISSUE HYPERTROPHY PERSIST BEYOND THE RESHAPING OF GUT MICROBIOTA IN YOUNG RATS FED A FRUCTOSE-RICH DIET. J NUTR BIOCHEM. 2022 Dec;:109247 WB ; Rat.
[IF=3.388] Xiao et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. (2017) Invest.Ophthalmol.Vis.Sci. 58:6123-6132 IHC ; Mouse.
[IF=2.336] Gu X et al. SPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway. Clin Exp Pharmacol Physiol. 2019 Jun 17. WB ; Human.
|
研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞粘附分子 G蛋白偶聯(lián)受體 內(nèi)皮細(xì)胞 細(xì)胞骨架 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse,Rat (predicted: Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 44 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human EDG1/CD363: 51-150/382 <Extracellular> |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Post-translational modifications: S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21453 Gene ID: 1901 Database links: Entrez Gene: 1901 Human Entrez Gene: 13609 Mouse SwissProt: P21453 Human SwissProt: O08530 Mouse 研究發(fā)現(xiàn),S1P1與VEGF肩并肩相互協(xié)作,從而促進(jìn)血管生長(zhǎng)。VEGF是很多不同抗癌藥物的作用靶標(biāo)。血管新生在很多疾病是異常的。靶向S1P1和VEGF可能要比只使用VEGF抑制劑更加有效地治療疾??;S1P1是一種關(guān)鍵性的血管新生反應(yīng)性基因。研究人員證實(shí)當(dāng)新的血管網(wǎng)絡(luò)形成時(shí),由此產(chǎn)生的血液流動(dòng)激活血管內(nèi)皮細(xì)胞表面上的S1P1,并把信號(hào)傳遞到這些細(xì)胞內(nèi)部從而讓新形成的血管網(wǎng)絡(luò)穩(wěn)定化。因此阻斷S1P1可能有助于切斷給癌性腫瘤提供資源的血液供應(yīng)。 |
產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 成熟妇人A片免费看网站 | 国产一级特黄aaa大片 | 在线无码观看免费网站入口 | 国产又猛又黑又粗又长 | 欧一美一色一伦一区二区三区 | 国产一级婬片永久免费看久久 | 色情六月丁香色情久久 | 亚洲欧美动漫偷拍 | 做爱视频免费在线观看 | 日韩在线视频免费观看 | 精品国产污软件网站免费入口 | 在线观看av网站 | 调教我的妺妺H肉yin荡视频 | 啊轻点灬大巴太粗太长www91 | 911人成网站色www | 国产精品免费一区二区 | 国精品无码一区二区三区在线秋菊 | 丰满爆乳一区二区三区霸乳 | 久久这里只有精品10 | 一级A片迷奷系列迷奷犯 | 国产精品岛国久久久久久 | 午夜福利视频在线播放 | 国产小视频在线播放 | 東北老熟女黃色A片 | 国产乱国产乱老熟 | 一本色道久久亚洲综合精品蜜桃 | 免费又黄又无码的视频 | 人人看九九人人做九九 | 国产一级特黄AAA大片 | 欧美性猛交xxx乱大交3 | 中文在线字幕观看 | 国产黄色大片在线观看 | 重口老女人乱视频.国产成人夜精 | 中文字幕在线免费观看 | 三人成全视频在线观看免费 | 丰满少妇毛片97级无遮挡 | 久久人妻少妇嫩草AV蜜桃漫画 | 国内毛片毛片毛片毛片 | 99久久久久成人国产免费 | 国产日韩精品无码去免费专区国产 |