| 產(chǎn)品編號 | bs-7518R |
| 英文名稱 | Ribonuclease Inhibitor Rabbit pAb |
| 中文名稱 | 核糖核酸酶抑制因子1抗體 |
| 別 名 | Placental ribonuclease inhibitor; RNH; RNH1; Placental RNase inhibitor; PRI; RAI; RI antibody Ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; Ribonuclease/angiogenin inhibitor; RINI_HUMAN; RNase inhibitor; RNH 1; Rnh1 ribonuclease/angiogenin |
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Specific References (1) | bs-7518R has been referenced in 1 publications.
[IF=1.6] Tang, Y., et al. "Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway." Clinical and Translational Oncology: 1-8. IHC-P ; Human.
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| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ribonuclease Inhibitor: 101-200/461 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis. Subunit: Forms high-affinity heterodimers with RNASE1, ANG and RNASE2. Subcellular Location: Cytoplasm. Post-translational modifications: The N-terminus is blocked. At least 30 of the 32 cysteine residues are in the reduced form. Similarity: Contains 15 LRR (leucine-rich) repeats. SWISS: P13489 Gene ID: 6050 Database links: Entrez Gene: 6050 Human Entrez Gene: 107702 Mouse Omim: 173320 Human SwissProt: P13489 Human SwissProt: Q91VI7 Mouse Unigene: 530687 Human
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| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control(blue): Hela cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Ribonuclease Inhibitor antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
