| 產(chǎn)品編號 | bs-2611R |
| 英文名稱 | IL17C Rabbit pAb |
| 中文名稱 | 白介素-17C抗體 |
| 別 名 | Interleukin-17C; IL17C; CX2; IL-17C; IL 17C; IL-21; IL 21; MGC126884; MGC138401; Cytokine CX2; IL17C_HUMAN. |
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Specific References (4) | bs-2611R has been referenced in 4 publications.
[IF=6.676] Takuya Takeichi. et al. Ceramide analysis in combination with genetic testing may provide a precise diagnosis for self-healing collodion babies. J LIPID RES. 2022 Nov;:100308 IHC ; Human.
[IF=5.9] Huang et al. IL-17C is required for lethal inflammation during systemic fungal infection. (2016) Cell.Mol.Immunol. 13:474-83 IHC ; Mouse.
[IF=4.99] Huang, Jinling, et al. "IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis." The Journal of Immunology (2016): 1600977. IHC-P ; Mouse.
[IF=2.952] Xiaoyan Pu. et al. Oxidative stress and expression of inflammatory factors in lung tissue of acute mountain sickness rats. Mol Med Rep. 2022 Feb;25(2):1-7 WB ; Rat.
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| 研究領域 | 免疫學 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-17C: 101-197/197 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. [provided by RefSeq]. Function: Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. Subcellular Location: Secreted. Similarity: Belongs to the IL-17 family. SWISS: Q9P0M4 Gene ID: 27189 Database links: Entrez Gene: 27189 Human Omim: 604628 Human SwissProt: Q9P0M4 Human Unigene: 278911 Human |
| 產(chǎn)品圖片 |
Sample:
Lane1: Lung(Rat) Lysate at 30 ug
Lane2: Pancreas(Rat) Lysate at 30 ug
Primary: Anti-IL-17C (bs-2611R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 20kD
Observed band size : 22kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL17C) Polyclonal Antibody, Unconjugated (bs-2611R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Human colon cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL17C Polyclonal Antibody, Unconjugated(bs-2611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
