| 產(chǎn)品編號 | bs-2444R |
| 英文名稱 | Pleiotrophin Rabbit pAb |
| 中文名稱 | 多效生長因子抗體 |
| 別 名 | PTN; HARP; HBBM; HBGAM; HBGF8; HBNF; HBNF1; Heparin Binding Growth Factor 8; Heparin binding neurite outgrowth promoting factor 1; heparin-binding growth associated molecule; NEGF1; Neurite Growth Promoting Factor 1; OSF-1; osteoblast-specific factor; PTN_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PTN: 101-168/168 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pleiotrophin is a heparin-binding growth factor involved in the differentiation and proliferation of neuronal tissue during embryogenesis, and also secreted by melanoma and breast carcinoma cells. Pleiotrophin exhibits mitogenic and angiogenic properties and has been shown to influence the vascular supply, expansion and metastasis of tumour cells. Function: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Subunit: Interacts with ALK and NEK6. Subcellular Location: Secreted. Tissue Specificity: Osteoblast and brain. Post-translational modifications: Phosphorylated by NEK6. Similarity: Belongs to the pleiotrophin family. SWISS: P21246 Gene ID: 5764 Database links: Entrez Gene: 5764 Human Entrez Gene: 19242 Mouse Omim: 162095 Human SwissProt: P21246 Human SwissProt: P63089 Mouse Unigene: 371249 Human Unigene: 279690 Mouse Unigene: 1653 Rat 多效生長因子(pleiotrophin）是一種分泌性的生長因子,具有促進腫瘤細胞的增殖與遷移，對腫瘤血管的生成有促進作用。 |
| 產(chǎn)品圖片 |
2675號盒-20230316-LLL_20.0x C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
2675號盒-20230316-LLL_20.0x C.jpg)
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
2675號盒-20230316-LLL_20.0x C.jpg)
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
