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[IF=8.101] Linlin Meng. et al. Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells. FREE RADICAL BIO MED. 2023 Jun;202:2  IHC ;  Mouse.  

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[IF=4.522] Fujino S et al. Expression and function of dopamine in odontoblasts. J Cell Physiol. 2019 Oct 15.  IHC-P&WB ;  Rat.  

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[IF=4.181] Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446.  WB ;  Mouse.  

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[IF=2.35] Xu, Jiao-jiao, et al. "Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells." Acta Pharmacologica Sinica (2014).  WB ;  Human.  

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[IF=0] Hardt, Olaf, et al. "SSEA4 AND ST3GAL2 AS CHEMOTHERAPEUTIC DRUG RESPONSE BIOMARKERS." U.S. Patent No. 20,150,316,556. 5 Nov. 2015.  FCM ;  Mouse.  

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Sample: Brain (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti- Dopamine D5 receptor (bs-1747R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53kD Observed band size: 53kD

Sample: Skin(Mouse) Lysate at 40 ug Skin(Rat) Lysate at 40 ug Primary: Anti-Dopamine D5 receptor (bs-1747R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD

Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- DRD5 antibody(bs-1747R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (bs-1747R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1747R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.