| 產(chǎn)品編號 | bsm-61754R |
| 英文名稱 | Mammaglobin A Recombinant Rabbit mAb |
| 中文名稱 | 乳腺珠蛋白1重組兔單抗 |
| 別 名 | Mammaglobin 1; Mammaglobin-A; MGB1; Scgb2a2; SG2A2; UGB2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human (predicted: Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 10 kDa |
| 檢測分子量 | 18 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mammaglobin A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SCGB2A2 Belongs to the secretoglobin family. Lipophilin subfamily. 2 isoforms of the human protein are produced by alternative splicing. SWISS: Q13296 Gene ID: 4250 乳腺球蛋白是一個乳腺相關糖蛋白，它是一個分泌素家族（包括子宮球蛋白和lipophilin）成員的遠親。與其他分泌素家族成員不同，乳腺球蛋白的mRNA在乳腺組織中特異表達。在石蠟組織切片的檢測中，該抗體檢測乳腺癌組織中的乳腺珠蛋白敏感度高達80%。當和其他乳腺特異標記如GCDFP-15聯(lián)合應用時，檢測乳腺珠蛋白敏感度高達84%。有資料表明檢測乳腺球蛋白，在鑒定原發(fā)癌是否發(fā)生轉(zhuǎn)移中起到至關重要的作用。 |
| 產(chǎn)品圖片 |
Western blot analysis of Human breast cancer lysate. Using Mammaglobin A (bsm-61754R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Mammaglobin A Monoclonal Antibody, Unconjugated(bsm-61754R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
